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pakt 5473  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc pakt 5473
    Pakt 5473, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 43 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pakt+5473/Phospho-Src+(Ser17)+Antibody/us12594421-308-43-46
    Average 94 stars, based on 43 article reviews
    pakt 5473 - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Generated:

    Article Title: Geranylgeranyltransferase I inhibitor for treatment of a PTEN defective cancer
    Article Snippet: .. The following antibodies were used: IP3R1(Bethyl no. A302-157A), IP3R-2(Millipore no. AB3000), IP3R3 (BD-Pharmingen no. 610312), PTEN (Cell Signaling Technology no. 9559S), calnexin (Santa Cruz no. sc11397), cleaved PARP (Cell Signaling Technology no. 5625S), cleaved caspase-3 (Cell Signaling Technology #9661S), cytochrome c (BD no. 556433), AKT (Cell Signaling Technology no. 2920S), pAKT-5473 (Cell Signaling Technology no. 4058S), GFP (Cell Signaling Technology no. 2956S), CUL1 (Invitrogen no. 718700), SKP1 (generated in-house), Flag (Sigma), HA (Covance), α-tubulin (Sigma), and β-actin (Sigma). .. Isotype-specific horseradish peroxidase conjugated secondary antibodies were used for detection by enhanced chemiluminescence (Pierce).

    Immunohistochemistry:

    Article Title: Compositions and methods for treating cancer
    Article Snippet: .. The following antibodies were used —GAPDH (GeneTex GTX627408, 1:5000), pERK1/2 T202/Y204 (CST 4370, 1:2000), total ERK1/2 (CST 4696, 1:2000), pAKT 5473 (CST 9271, 1:1000), pPAK1 T423/pPAK2 T402 (CST 2601S, 1:1000), PAK1/2/3 (CST 2604, 1:2000), PAK1 (CST 2602, 1:1000), pPAK4 S474/pPAK5 S602/pPAK6 5560 (CST 3241, 1:1000), PAK4 (CST 62690, 1:1000), anti-mouse Eu (Molecular Devices R8205), anti-rabbit Eu (Molecular Devices R8204), anti-mouse-HRP (CST 7076, 1:5000), and anti-rabbit-HRP (CST 7074, 1:5000), Ki67 (Abcam, IHC 1:100), cleaved caspase 3 (Asp175, CST 9661, IHC 1:100), goat anti-rabbit Alexa Fluor 568 (Abcam, IF 1:500). .. Fluorescent phalloidins and DAPI were from Invitrogen.

    Electrophoresis:

    Article Title: Compositions and methods for increasing cancer cell sensitivity to alternating electric fields
    Article Snippet: Samples were run on SDS-polyacrylamide gel electrophoresis (Bolt 8% Bis-Tris base gel NW00080BOX, Thermo-Fischer). .. After electrophoresis, proteins were transferred to 0.2 μm polyvinylidene difluoride membrane (Immuno-Blot PVDF #162-0177, Bio-Rad) and probed with the appropriate primary antibody: GAPDH (SC-32233, Santa Cruz), Vinculin (ab140007, Abcam), Cyclin D1 (2922, Cell Signaling), Rb (9309, Cell Signaling), pRb S807/811 (8516, Cell Signaling), pAkt 5473, (4051, Cell signaling), Akt (2920, Cell signaling)), FAK (3283S, Cell signaling), Phospho-FAK (Tyr397) (3283S, Cell signaling, Src (2109S, cell signaling) and Phospho-Src (Tyr416) (2101S, Cell signaling). followed by horseradish peroxidase-conjugated secondary antibody (goat anti rabbit 7074, Cell Signaling and goat anti mouse 7076, Cell Signaling) and a chemiluminescent substrate (WBLUF0100, Signa-Aldrich). ..

    Membrane:

    Article Title: Compositions and methods for increasing cancer cell sensitivity to alternating electric fields
    Article Snippet: Samples were run on SDS-polyacrylamide gel electrophoresis (Bolt 8% Bis-Tris base gel NW00080BOX, Thermo-Fischer). .. After electrophoresis, proteins were transferred to 0.2 μm polyvinylidene difluoride membrane (Immuno-Blot PVDF #162-0177, Bio-Rad) and probed with the appropriate primary antibody: GAPDH (SC-32233, Santa Cruz), Vinculin (ab140007, Abcam), Cyclin D1 (2922, Cell Signaling), Rb (9309, Cell Signaling), pRb S807/811 (8516, Cell Signaling), pAkt 5473, (4051, Cell signaling), Akt (2920, Cell signaling)), FAK (3283S, Cell signaling), Phospho-FAK (Tyr397) (3283S, Cell signaling, Src (2109S, cell signaling) and Phospho-Src (Tyr416) (2101S, Cell signaling). followed by horseradish peroxidase-conjugated secondary antibody (goat anti rabbit 7074, Cell Signaling and goat anti mouse 7076, Cell Signaling) and a chemiluminescent substrate (WBLUF0100, Signa-Aldrich). ..

    Western Blot:

    Article Title: Markers of triple-negative breast cancer and uses thereof
    Article Snippet: .. Immunoblotting Cells were trypsinized, lysed and relative protein expression was determined by Western blot as previously described (JCI reference) with the following antibodies; the AR polyclonal antibody, SC-N20 (Santa Cruz Biotechnology), pS6-Ser235/236 (Cell Signaling #4856), S6 (Cell Signaling #2317), pAKT-5473 (Cell Signaling #9271), AKT (Cell Signaling #9272), PARP (Cell Signaling #9542) and GAPDH (Millipore). ..

    Expressing:

    Article Title: Markers of triple-negative breast cancer and uses thereof
    Article Snippet: .. Immunoblotting Cells were trypsinized, lysed and relative protein expression was determined by Western blot as previously described (JCI reference) with the following antibodies; the AR polyclonal antibody, SC-N20 (Santa Cruz Biotechnology), pS6-Ser235/236 (Cell Signaling #4856), S6 (Cell Signaling #2317), pAKT-5473 (Cell Signaling #9271), AKT (Cell Signaling #9272), PARP (Cell Signaling #9542) and GAPDH (Millipore). ..

    other:

    Article Title: Therapeutic combinations using IGF1R pathway inhibitors, and methods to predict anti-IGF1R therapeutic efficacy
    Article Snippet: Samples for immunoblot analysis were collected using RIPA buffer (50 mM Tris pH 7.4, 150 mM NaCl, 1 mM EDTA, 0.5% Nonidet P-40, 0.5% NaDeoxycholate, 0.1% SDS, 1×HALT cocktail [Thermo Fisher #78442]) and standard immunoblot technique was followed.



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